Research Paper Volume 13, Issue 8 pp 12160—12178

Balasubramide derivative 3C attenuates atherosclerosis in apolipoprotein E-deficient mice: role of AMPK-STAT1-STING signaling pathway

3C inhibits IFN-γ stimulated JAK2-STAT1 signaling and downstream signaling in AMPK dependent manner. (A) IFN-γ time-dependently induce phosphorylation of Stat1 and initiate downstream STING-IRF3 signaling. (B) Quantitative results for the relative level of STING and P-IRF3 normalized to β-Actin (n=3 per group). (C) 3C activate AMPK and inhibit IFN-γ stimulated Jak-Stat1 and downstream STING-IRF3 signaling. (D) Relative level of STING, P-IRF3 normalized to β-Actin (n=3 per group). (E) Relative gene expression of STING, IFN-α, IFN-β normalize to β-Actin. Results are expressed as means ± SEM (n = 3). *P**P

Figure 6. 3C inhibits IFN-γ stimulated JAK2-STAT1 signaling and downstream signaling in AMPK dependent manner. (A) IFN-γ time-dependently induce phosphorylation of Stat1 and initiate downstream STING-IRF3 signaling. (B) Quantitative results for the relative level of STING and P-IRF3 normalized to β-Actin (n=3 per group). (C) 3C activate AMPK and inhibit IFN-γ stimulated Jak-Stat1 and downstream STING-IRF3 signaling. (D) Relative level of STING, P-IRF3 normalized to β-Actin (n=3 per group). (E) Relative gene expression of STING, IFN-α, IFN-β normalize to β-Actin. Results are expressed as means ± SEM (n = 3). *P<0.05, **P<0.01.