Figure 5. Immunohistochemical staining analyses of ULBP2 expression in COAD tumor tissues and adjacent normal tissues. (A–D) ULBP2 staining results were observed in the cytoplasm of colon cells; (A) Positive staining of COAD tumor tissues; (B) Negative staining of COAD tumor tissues; (C) Positive staining of adjacent normal colon tissues; (D) Negative staining of adjacent normal tissues; (E, F) Diagnostic analysis of ULBP2 expression in COAD: (E) Scatter plot; (F) Diagnostic ROC curve; survival analysis of ULBP2 expression in COAD: (G, H) Kaplan–Meier survival curve of ULBP2 protein expression in COAD OS and RFS; (I, J) Stratified analysis of ULBP2 expression in different clinical parameter layers in COAD patients: (A) OS; (B) RFS. Notes: COAD, colon adenocarcinoma; ULBP: unique long 16 (UL16)-binding protein; ROC, receiver operating characteristic; AUC: area under the curve; CI: confidence interval; OS, overall survival; RFS, recurrence-free survival. Magnification, 200×.