Figure 4. Effects of age on SGN density in marmosets. (A–A”) Confocal whole-mount immunofluorescence images of the middle turn of a cochlea in a 2-year-old marmoset. (A) Hair cells labeled with MYO7A. (A’) Supporting cells positive for SOX2. (A”) Neural fibers labeled with NF-H. (A”’) Merged channels figure, MYO7A (green), SOX2 (red), NF-H (magenta), DAPI (blue). (B–B”) Confocal images of a cochlear cryosection from a 2-year-old marmoset. SGNs labeled with TUJ. TUJ-positive neurofilaments connect the basilar membrane (BM) to the modiolus. (C–C”) Confocal images of a cochlear cryosection from a 12-year-old marmoset. Scale bar: 100 μm.