Figure 3. Screening of 20 differentially expressed genes for anti-HCC effects in vitro. (A, B) The 20 genes most down-regulated by morphine treatment were knocked down using lentivirus-delivered shRNA, and effects on QGY-7703 cell proliferation were examined. A) Shows the cells number after the 20 genes were knock down. B) Shows the cells proliferation fold changed after the 20 genes were knock down. (C–E) Cells were treated with a plasmid encoding a negative-control shRNA (shCtrl) or plasmids encoding shRNAs targeting PNO1 (shPNO1) or DDX49 (shDDX49). shPC as the protooncogene X specific-targeting shRNA. Then the cells were analyzed for five days by fluorescence microscopy, and the data were used to measure proliferation. Scale bar, 50 μm. *P < 0.01 compare to the control group.