Figure 5. DDX49 expression after morphine treatment and its impact on MAPK signaling. QGY-7703 and SMMC-7721 cells were treated with morphine (10μM) or saline for 48 h. (A, B) Effect of morphine (10μM) on levels of DDX49 in QGY-7703 and SMMC-7721 cells. (C, D) Effect of DDX49 expression on levels of phosphorylated MAPK (P-MAPK) and levels of total MAPK (T-MAPK). *P < 0.01 compared to the respective control group. CON cells were derived cells without any treating. N- CON cells were knock down using negative control vector. DDX49-KD cells were knock down of DDX49 using lentivirus.