Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis

CircRNA

Figure 1. CircRNA_30032 expression is upregulated in kidney tissues of UUO-induced C57BL/6 mice and TGF-β1 treated BUMPT cells. (A) The heat map shows the expression of circRNAs in the kidney tissues of sham and UUO group mice on days 3 and 7. (B) The co-upregulation amount of circRNAs at (more than 2 fold changes) days 3 and 7 in UUO group vs. Sham group. (C) The sequence name and fold change values of significantly upregulated circRNAs (> 3 fold) in the kidney tissues of UUO group mice on days 3 and 7. (D) RNA-FISH images show intracellular localization of circRNA_30032 in BUMPT cells treated with saline or 5 ng/ml TGF-β1 for 24 h. (E, F) RT-qPCR analysis shows expression levels of circRNA_30032 in BUMPT cells treated with saline or 5ng/ml TGF-β1 for 6, 12 and 24 h and kidney tissues of sham and UUO group mice on days 3 and 7. Note: The data are expressed as means ± SD (n = 6); # denotes p < 0.05 for TGF-β1 groups at 12 and 24 h vs. saline group and UUO groups at days 3 and 7 vs. sham group.