Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis

CircRNA

Figure 10. CircRNA_30032 siRNA ameliorates UUO-induced expression of collagen I, collagen III and fibronectin by targeting miR-96-5p/HBEGF/KRAS axis. (A, B) RT-qPCR analysis shows (A) circRNA_30032 and (B) miR-96-5p levels in the kidney tissues harvested from UUO and sham group mice injected with scrambled siRNA or circRNA_30032 siRNA. (C) Representative western blot images and (DH) densitometric measurements show the levels of (C, D) collagen I, (C, E) collagen III, (C, F) fibronectin, (C, G) HBEGF, and (C, H) KRAS proteins in the kidney tissues harvested from UUO and sham group mice injected with scrambled siRNA or circRNA_30032 siRNA. GAPDH was used as a loading control. Note: The data are expressed as means ± SD (n =6). # denotes p < 0.05 when comparing circRNA_30032 siRNA plus sham group or scrambled siRNA plus UUO group vs. scrambled siRNA plus sham group; * denotes p<0.05 when comparing circRNA_30032 siRNA plus UUO group vs. scrambled siRNA plus UUO group.