Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis

TGF-β1 induces CircRNA

Figure 2. TGF-β1 induces CircRNA_30032 in a p38MAPK-dependent manner in BUMPT cells. (A, F) RT-qPCR analysis shows relative levels of circRNA_30032 expression in BUMPT cells treated with 5ng/mL TGF-β1 with or without p38MAPK inhibitor or agonist. (B, G) Western blot images and (CE and HJ) quantitative analysis of p-p38MAPK, p38MAPK and GAPDH (loading control) protein levels in BUMPT cells treated with 5ng/mL TGF-β1 with or without p38MAPK inhibitor/ agonist. Note: The data are expressed as means ± SD (n = 6). # denotes p < 0.05 while comparing TGF-β1 and TGF-β1/p38MAPK inhibitor or agonist treatment groups vs. scramble group; * denotes p < 0.05 when comparing p38MAPK inhibitor or agonist /TGF-β1 group vs. TGF-β1 group.