Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis

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Figure 6. MiR-96-5p inhibits TGF-β1-induced expression of collagen I, collagen III, and fibronectin. (A) RT-qPCR analysis shows miR-96-5p expression levels in BUMPT cells transfected with 100 nM miR-96-5p mimics or scrambled miRNA, and then treated with or without TGF-β1 for 24h. (B) Representative western blot images and (CE) densitometric measurements show (B, C) collagen I, (B, D) collagen III, and (B, E) fibronectin protein levels in BUMPT cells transfected with 100 nM miR-96-5p mimics or scrambled miRNA, and then treated with or without TGF-β1 for 24h. Note: The data are expressed as means ±SD (n = 6). # denotes p < 0.05 when comparing scrambled miRNA plus TGF-β1 group or miR-96-5p mimics plus saline group vs. scrambled miRNA plus saline group; * denotes p<0.05 when comparing miR-96-5p mimics plus TGF-β1group vs. scrambled miRNA plus TGF-β1 group.