Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis

CircRNA

Figure 9. CircRNA_30032 silencing attenuates UUO-induced renal fibrosis in male C57BL/6 mice. (A) H&E and (B) Masson staining images of kidney tissue sections. (CE) Representative immunohistochemical (IHC) staining images show expression patterns of (C) collagen I, (D) collagen III, and (E) fibronectin in the kidney tissue sections of control and circRNA-30032 silenced UUO and sham group mice. (F) Quantification of fibrotic area (%) in the kidney cortex of control and circRNA-30032 silenced UUO and sham group mice based on Masson staining. (G) Quantitative analysis of IHC data shows collagen I, collagen III, and fibronectin levels in the kidney tissue sections of control and circRNA-30032 silenced UUO and sham group mice. Note: magnification, ×200; scale bar = 50μm. The data are expressed as means ± SD (n=6). # denotes p < 0.05 when comparing circRNA_30032 siRNA plus sham group or scrambled siRNA plus UUO group vs. scrambled siRNA plus sham group; * denotes p<0.05 when comparing circRNA_30032 siRNA plus UUO group vs. scrambled siRNA plus UUO group.