Research Paper Volume 13, Issue 9 pp 12800—12816

Internal modulation of proteolysis in vascular extracellular matrix remodeling: role of ADAM metallopeptidase with thrombospondin type 1 motif 5 in the development of intracranial aneurysm rupture

Recombinant protein ADAMTS-5 protected elastase-induced IA mice from vascular matrix degradation. (A) Representative hematoxylin and eosin (H&E) and elastin Verhoeff-Van Gieson (EVG)-stained section of cerebral arterial sections of sham mice, control (CTRL) IA mice, and recombinant protein ADAMTS-5-administered (rADAMTS-5) IA mice. Scale bar: 20 μm. (B) Quantification histogram showing elastin filament degradation. Arterial wall elastin filament degradation was evaluated based on the degree of degradation in elastin fibers (graded 0–4) as described in the Materials and Methods. **PC) Picrosirius red staining for collagen content within the arteries captured by polarization microscopy imaging from the sham mice, control IA mice, and recombinant protein ADAMTS-5-administered (rADAMTS-5) IA mice. Compared with the control group, the rADAMTS-5-administered group showed increased collagen birefringence under polarization. Scale bar: 20 μm.

Figure 3. Recombinant protein ADAMTS-5 protected elastase-induced IA mice from vascular matrix degradation. (A) Representative hematoxylin and eosin (H&E) and elastin Verhoeff-Van Gieson (EVG)-stained section of cerebral arterial sections of sham mice, control (CTRL) IA mice, and recombinant protein ADAMTS-5-administered (rADAMTS-5) IA mice. Scale bar: 20 μm. (B) Quantification histogram showing elastin filament degradation. Arterial wall elastin filament degradation was evaluated based on the degree of degradation in elastin fibers (graded 0–4) as described in the Materials and Methods. **P<0.01. (C) Picrosirius red staining for collagen content within the arteries captured by polarization microscopy imaging from the sham mice, control IA mice, and recombinant protein ADAMTS-5-administered (rADAMTS-5) IA mice. Compared with the control group, the rADAMTS-5-administered group showed increased collagen birefringence under polarization. Scale bar: 20 μm.