Research Paper Volume 13, Issue 9 pp 12800—12816

Internal modulation of proteolysis in vascular extracellular matrix remodeling: role of ADAM metallopeptidase with thrombospondin type 1 motif 5 in the development of intracranial aneurysm rupture

Inhibition of ADAMTS-5 causes intracranial hemorrhage in zebrafish. (A) Zebrafish embryos treated with DMSO (control, CTRL group), and intracranial hemorrhage in embryos treated with 0.5 μg/mL of ADAMTS-5 inhibitor (ATS5-in). The black arrow indicates the bleeding site. (B) O-Dianisidine staining confirming intracranial hemorrhage at 72 hpf. (C) Confocal microscopy showing cerebral blood vessel leakage of the Tg (flk: EGFP; gata1: DsRed) embryo. (D) Incidence rates of intracranial hemorrhage of the control group (CTRL) and ADAMTS-5 inhibitor treatment group (ATS5-in) at 0.25 μg/mL and 0.5 μg/mL. Scale bar: 50 μm. **P

Figure 7. Inhibition of ADAMTS-5 causes intracranial hemorrhage in zebrafish. (A) Zebrafish embryos treated with DMSO (control, CTRL group), and intracranial hemorrhage in embryos treated with 0.5 μg/mL of ADAMTS-5 inhibitor (ATS5-in). The black arrow indicates the bleeding site. (B) O-Dianisidine staining confirming intracranial hemorrhage at 72 hpf. (C) Confocal microscopy showing cerebral blood vessel leakage of the Tg (flk: EGFP; gata1: DsRed) embryo. (D) Incidence rates of intracranial hemorrhage of the control group (CTRL) and ADAMTS-5 inhibitor treatment group (ATS5-in) at 0.25 μg/mL and 0.5 μg/mL. Scale bar: 50 μm. **P<0.01.