Research Paper Volume 13, Issue 9 pp 12817—12832

Hydroxyurea-induced membrane fluidity decreasing as a characterization of neuronal membrane aging in Alzheimer’s disease

Validation of membrane aging in APP/PS1-mutated transgenic mice. In the dissected cortical region, APP/PS1-mutated transgenic mice showed accelerated Aβ deposition after HU intracerebroventricular injection compared with mice with NS injection and wild-type controls. Scale bar: 1 mm (A). For the membrane fluidity determination probed with TMA-DPH and DPH, 32μM HU injection dramatically increased r values in the hippocampal and cortical membranes of APP/PS1 mice compared with NS-injected ones (n = 3, P n = 3, P B–E). All the data are expressed as mean ± SD from three independent experiments (N = 3). *P

Figure 3. Validation of membrane aging in APP/PS1-mutated transgenic mice. In the dissected cortical region, APP/PS1-mutated transgenic mice showed accelerated Aβ deposition after HU intracerebroventricular injection compared with mice with NS injection and wild-type controls. Scale bar: 1 mm (A). For the membrane fluidity determination probed with TMA-DPH and DPH, 32μM HU injection dramatically increased r values in the hippocampal and cortical membranes of APP/PS1 mice compared with NS-injected ones (n = 3, P < 0.05). Same trends were found only in hippocampal membranes in wild-type controls (n = 3, P < 0.05) (BE). All the data are expressed as mean ± SD from three independent experiments (N = 3). *P < 0.05. Two-way ANOVA was used to determine the statistical significance of the differences.