Figure 5. Membrane aging increased Aβ aggregation in vitro. The results of immunostaining with 6E10 showed that membrane aging significantly increased the number of positively stained neurons compared with the control (P < 0.01). After co-treatment with DNA, Aβ aggregation decreased (A, B). Further quantification of Aβ in vitro was done by ELISA detection. The average concentration of Aβ1-40 was at least 2 pmol/L higher in the membrane aging group compared with the control group (C). However, Aβ1-42 was under the detection threshold, which was hard to quantify. All the data are expressed as mean ± SD from three independent experiments (N = 3). *P < 0.05, **P < 0.01. The Student t test was used to determine the statistical significance of the differences.