Figure 4. FEZF1-AS1 negatively regulated the expression of miR-107. (A) The putative binding sites of miR-107 in FEZF1-AS1. (B, C) The relative luciferase activity was evaluated by luciferase gene reporter assay following cell transfection with pmirGlo-FEZF1-AS1-WT (wild type) or pmirGLo-FEZF1-AS1-MUT (mutant type) and miR-107 mimic or mimic-NC into SNU-398 and SNU-449 cells, *P < 0.05. (D) The expression of miR-107 was assessed after downregulation of FEZF1-AS1 in SNU-398 and SNU-449 cells by qRT PCR, *P < 0.05. (E) The expression of miR-107 was tested after overexpression of FEZF1-AS1 by q-RT-PCR, *P < 0.05.