Research Paper Volume 13, Issue 9 pp 12955—12972

PINK1/Parkin-mediated mitophagy inhibits warangalone-induced mitochondrial apoptosis in breast cancer cells

Warangalone decreases the viability of breast cancer cells. (A) MDA-MB-231, MCF7, SKBR3, ZR75-1 and MCF-10A cells were treated with warangalone of different concentrations for 12, 24, and 48 h. Cell viability was detected by MTT assay. One-way ANOVA was used to compare between the control and warangalone treatment groups (n ≥ 3). *P P P B) MDA-MB-231/MCF7 cells were treated with 0, 10, 20 and 30 μM warangalone for 24 h. Cell morphology was observed by microscopy.

Figure 1. Warangalone decreases the viability of breast cancer cells. (A) MDA-MB-231, MCF7, SKBR3, ZR75-1 and MCF-10A cells were treated with warangalone of different concentrations for 12, 24, and 48 h. Cell viability was detected by MTT assay. One-way ANOVA was used to compare between the control and warangalone treatment groups (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 compared to the respective control group. (B) MDA-MB-231/MCF7 cells were treated with 0, 10, 20 and 30 μM warangalone for 24 h. Cell morphology was observed by microscopy.