Figure 9. Effect of HBOT on autophagy in cardiomyocytes of pre-diabetic rats after induction of aging by D-gal. (A) Beclin-1 expression. (B) p62 expression. (C) LC3II expression. (D) Representative images of western blotting bands. NDV, normal diet fed rats with vehicle; NDDg, normal diet fed rats with D-gal; HFV, high-fat diet fed rats with vehicle; HFDg, high-fat diet fed rats with D-gal; p62, Sequestosome-1 (ubiquitin-binding protein); LC3II, microtubule associated light chain 3II; HBOT, hyperbaric oxygen therapy. (n = 5/group). *P < 0.05.