Research Paper Volume 13, Issue 10 pp 13764—13787

LncRNAs induce oxidative stress and spermatogenesis by regulating endoplasmic reticulum genes and pathways

LncRNA and mRNA profiles based on RNA sequencing data. (A) Comparison of two-dimensional hierarchical clustering of distinguishable lncRNA expression profiles in individuals with oligozoospermia and controls. Red indicates high expression, green indicates low expression. Probes are shown in rows, and samples are shown in columns. (B) Two-dimensional hierarchical clustering of distinguishable mRNA expression profiles. (C) Volcano plot of differentially expressed lncRNAs in individuals with oligozoospermia compared with normal controls. Red points represent downregulated lncRNAs and yellow points represent upregulated lncRNAs in individuals with oligozoospermia with a greater than 2.0-fold change. (D) Volcano plot of differentially expressed mRNAs. (E) Violin plot of lncRNA and mRNA profiles in individuals with oligozoospermia. (F) t-distributed stochastic neighbor embedding (t-SNE) plot of samples based on lncRNA and mRNA profiles in individuals with oligozoospermia.

Figure 3. LncRNA and mRNA profiles based on RNA sequencing data. (A) Comparison of two-dimensional hierarchical clustering of distinguishable lncRNA expression profiles in individuals with oligozoospermia and controls. Red indicates high expression, green indicates low expression. Probes are shown in rows, and samples are shown in columns. (B) Two-dimensional hierarchical clustering of distinguishable mRNA expression profiles. (C) Volcano plot of differentially expressed lncRNAs in individuals with oligozoospermia compared with normal controls. Red points represent downregulated lncRNAs and yellow points represent upregulated lncRNAs in individuals with oligozoospermia with a greater than 2.0-fold change. (D) Volcano plot of differentially expressed mRNAs. (E) Violin plot of lncRNA and mRNA profiles in individuals with oligozoospermia. (F) t-distributed stochastic neighbor embedding (t-SNE) plot of samples based on lncRNA and mRNA profiles in individuals with oligozoospermia.