Research Paper Volume 13, Issue 10 pp 13788—13806

Redd1 knockdown prevents doxorubicin-induced cardiac senescence

Redd1 expression activates NF-κB signaling in senescent cardiomyocytes. (A, B) Western blot analysis of total and phosphorylated p65 and p38 MAPK in Dox-treated H9c2 cardiomyocytes following Redd1 overexpression and knockdown. (C) Immunofluorescent detection of nuclear translocation of p65 in Dox-treated H9c2 cardiomyocytes following Redd1 overexpression and knockdown. (D) Quantification of data from p65 nuclear translocation assays (n = 4 per group). *p *p &p

Figure 5. Redd1 expression activates NF-κB signaling in senescent cardiomyocytes. (A, B) Western blot analysis of total and phosphorylated p65 and p38 MAPK in Dox-treated H9c2 cardiomyocytes following Redd1 overexpression and knockdown. (C) Immunofluorescent detection of nuclear translocation of p65 in Dox-treated H9c2 cardiomyocytes following Redd1 overexpression and knockdown. (D) Quantification of data from p65 nuclear translocation assays (n = 4 per group). *p < 0.05 vs. Lv-shNC group. Data are mean ± SEM. *p < 0.05 vs. control group. &p < 0.05 vs. Lv-Redd1 group.