Research Paper Volume 13, Issue 10 pp 13846—13858

Esophageal carcinoma cell-excreted exosomal uc.189 promotes lymphatic metastasis

Exosomal uc.189 activated EPHA2/P38MAPK/VEGF-C pathways in HLECs. (A) mRNA levels of EPHA2 were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with the negative control (NC) by qRT-PCR. (B) mRNA levels of P38MAPK were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by qRT-PCR. (C) mRNA levels of VEGF-C were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by qRT-PCR. (D–F) Protein levels of EPHA2, P38MAPK, and VEGF-C were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by western blot. Error bars represent the mean ± SD of three independent experiments. *P

Figure 6. Exosomal uc.189 activated EPHA2/P38MAPK/VEGF-C pathways in HLECs. (A) mRNA levels of EPHA2 were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with the negative control (NC) by qRT-PCR. (B) mRNA levels of P38MAPK were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by qRT-PCR. (C) mRNA levels of VEGF-C were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by qRT-PCR. (D–F) Protein levels of EPHA2, P38MAPK, and VEGF-C were detected in HELCs of exosomal uc.189-overexpressing or exosomal uc.189-knockdown compared with NC by western blot. Error bars represent the mean ± SD of three independent experiments. *P < 0.05.