Research Paper Volume 13, Issue 10 pp 13876—13897

Prognostic value of members of NFAT family for pan-cancer and a prediction model based on NFAT2 in bladder cancer

NFAT2 participates in the regulation of BLCA as an oncogene. (A) The expression of NFAT2 was verified by WB. (B) The expression of NFAT2 was verified by RT-PCR. (C) The knockdown efficiency was verified by RT-PCR. (D) The knockdown efficiency was verified by WB. (E) The cell viability was assessed by cell proliferation assay (see Materials and Methods). (F) Cell proliferation detected by colony formation assay. (G) Cell proliferation was measured by EdU incorporation assay. (H) Cell invasion measured by Transwell migration assay. (I) Cell migration evaluated by wounded cell monolayer closure assay. (J) Western Blot analysis of protein expression of NFAT2 and EMT-linked gene products. (K) Quantitative RT-PCR analysis of mRNA for NFAT2 and EMT-related genes. (L) Tumor weight from control and NFAT2 knockdown mouse tumor groups. (M) Tumor volume from control and NFAT2 knockdown mouse tumor groups. (N) Tumor cell proliferation evaluated by Ki-67 immunohistochemical staining. *PPPP

Figure 13. NFAT2 participates in the regulation of BLCA as an oncogene. (A) The expression of NFAT2 was verified by WB. (B) The expression of NFAT2 was verified by RT-PCR. (C) The knockdown efficiency was verified by RT-PCR. (D) The knockdown efficiency was verified by WB. (E) The cell viability was assessed by cell proliferation assay (see Materials and Methods). (F) Cell proliferation detected by colony formation assay. (G) Cell proliferation was measured by EdU incorporation assay. (H) Cell invasion measured by Transwell migration assay. (I) Cell migration evaluated by wounded cell monolayer closure assay. (J) Western Blot analysis of protein expression of NFAT2 and EMT-linked gene products. (K) Quantitative RT-PCR analysis of mRNA for NFAT2 and EMT-related genes. (L) Tumor weight from control and NFAT2 knockdown mouse tumor groups. (M) Tumor volume from control and NFAT2 knockdown mouse tumor groups. (N) Tumor cell proliferation evaluated by Ki-67 immunohistochemical staining. *P<0.05; **P<0.01; ***P<0.001; #P<0.05. All data are representative of three independent experiments.