Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

Temporal patterns of neutrophils in peripheral blood and brain after SAH. (A) Representative FACS of blood from sham or SAH mice in different temporal patterns and the percentage of PMNs. (B) Representative photographs of immunofluorescence staining showing neutrophils with Ly6G (green) in cortex from sham or SAH mice in different temporal patterns. (C) Quantitative analyses of the percentage of PMNs from blood in different temporal patterns. n = 6/group. *p #p D) Quantitative analyses of the percentage of neutrophils from cortex in different temporal patterns. n = 6/group. Scale bar = 50 μm. *p #p

Figure 3. Temporal patterns of neutrophils in peripheral blood and brain after SAH. (A) Representative FACS of blood from sham or SAH mice in different temporal patterns and the percentage of PMNs. (B) Representative photographs of immunofluorescence staining showing neutrophils with Ly6G (green) in cortex from sham or SAH mice in different temporal patterns. (C) Quantitative analyses of the percentage of PMNs from blood in different temporal patterns. n = 6/group. *p < 0.05 vs sham, #p < 0.05 vs SAH-12 h. (D) Quantitative analyses of the percentage of neutrophils from cortex in different temporal patterns. n = 6/group. Scale bar = 50 μm. *p < 0.05 vs sham, #p < 0.05 vs SAH-24 h.