Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

Effect of neutrophils in brain after SAH. (A) Modified Garcia Score from sham or SAH mice in different temporal patterns. (B) Quantitative analyses of the percentage of neutrophils from cortex in different temporal patterns. n = 6/group. *p C) One to one correspondence diagram of Modified Garcia Score and the percentage of neutrophils from cortex. (D) There is a positive correlation between Modified Garcia Score and the percentage of neutrophils from cortex (p E) Representative FACS of blood from SAH mice treated 2 days previously, without or with anti-Ly6G Ab (1A8) and the percentage of PMNs. (F) Representative photograph showed the neutrophils with Ly6G (green) in different groups. n = 5/group. Scale bar = 50 μm. (G) Quantification of Modified Garcia Score at 24 h after SAH. (H) Quantification of Behavior Score at 24 h after SAH. n = 6/group. *P

Figure 4. Effect of neutrophils in brain after SAH. (A) Modified Garcia Score from sham or SAH mice in different temporal patterns. (B) Quantitative analyses of the percentage of neutrophils from cortex in different temporal patterns. n = 6/group. *p < 0.05 vs sham. (C) One to one correspondence diagram of Modified Garcia Score and the percentage of neutrophils from cortex. (D) There is a positive correlation between Modified Garcia Score and the percentage of neutrophils from cortex (p < 0.001). (E) Representative FACS of blood from SAH mice treated 2 days previously, without or with anti-Ly6G Ab (1A8) and the percentage of PMNs. (F) Representative photograph showed the neutrophils with Ly6G (green) in different groups. n = 5/group. Scale bar = 50 μm. (G) Quantification of Modified Garcia Score at 24 h after SAH. (H) Quantification of Behavior Score at 24 h after SAH. n = 6/group. *P < 0.05 versus SAH + vehicle group.