Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

The effect of the formation of NETs after SAH. (A) Representative photographs of immunofluorescence staining showing NETs (marked with CitH3 (red)) in neutrophil (marked with Ly6G (green)) in sham group and SAH 24 h group. (B) Quantitative analysis of CitH3 positive neutrophils. n = 5/group (C) Representative photographs of immunofluorescence staining showing pro-inflammatory subtype (marked with CD16/32 (red)) microglia (marked with IBA-1 (green)) in sham group and SAH 24 h group. (D) Quantitative analysis of CD16/32 positive microglia. n = 5/group. Scale bar = 50 μm. *P E) The quantification of SAH grade. n = 17/group. (F) Typical brains without or with SAH. (G) Quantification of Modified Garcia Score at 24 h after SAH. n = 17/group. (H) Quantification of Behavior Score at 24 h after SAH. n = 17/group. (I) Quantification of brain water content. n = 6/group. (J) Quantitative analysis of FJC staining. n = 5/group. (K) Representative photograph showed the FJC positive cell (green) in different groups. Scale bar = 50 μm. *P #P

Figure 5. The effect of the formation of NETs after SAH. (A) Representative photographs of immunofluorescence staining showing NETs (marked with CitH3 (red)) in neutrophil (marked with Ly6G (green)) in sham group and SAH 24 h group. (B) Quantitative analysis of CitH3 positive neutrophils. n = 5/group (C) Representative photographs of immunofluorescence staining showing pro-inflammatory subtype (marked with CD16/32 (red)) microglia (marked with IBA-1 (green)) in sham group and SAH 24 h group. (D) Quantitative analysis of CD16/32 positive microglia. n = 5/group. Scale bar = 50 μm. *P < 0.05 versus Sham. (E) The quantification of SAH grade. n = 17/group. (F) Typical brains without or with SAH. (G) Quantification of Modified Garcia Score at 24 h after SAH. n = 17/group. (H) Quantification of Behavior Score at 24 h after SAH. n = 17/group. (I) Quantification of brain water content. n = 6/group. (J) Quantitative analysis of FJC staining. n = 5/group. (K) Representative photograph showed the FJC positive cell (green) in different groups. Scale bar = 50 μm. *P < 0.05 versus Sham, #P < 0.05 versus SAH + vehicle group.