Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

Inhibiting the formation of NETs prevents pro-inflammatory subtype transition of microglia. (A) Representative photograph showed the co-localization of CitH3 positive cell (red) with Ly6G (green), NE positive cell (red) with MPO (green), CD16/32 positive cell (red) with IBA-1 (green), IL1β positive cell (red) with IBA-1 (green), in different groups. (B) Quantitative analysis of CitH3 positive neutrophil in different groups. n = 5/group. (C) Quantitative analysis of NE and MPO positive cells in different groups. n = 5/group. (D) Quantitative analysis of CD16/32 positive microglia in different groups. n = 5/group. (E) Quantitative analysis of IL1β positive microglia in different groups. n = 5/group. (F) Representative western blotting images of TNF-α expression in cortex in different groups. (G) Quantitative analysis of TNF-α expression in cortex in different groups. n = 6/group. (H) Representative western blotting images of IL1β expression in cortex in different groups. (I) Quantitative analysis of IL1β expression in cortex in different groups. n = 6/group. Scale bar = 50 μm. *P #P

Figure 6. Inhibiting the formation of NETs prevents pro-inflammatory subtype transition of microglia. (A) Representative photograph showed the co-localization of CitH3 positive cell (red) with Ly6G (green), NE positive cell (red) with MPO (green), CD16/32 positive cell (red) with IBA-1 (green), IL1β positive cell (red) with IBA-1 (green), in different groups. (B) Quantitative analysis of CitH3 positive neutrophil in different groups. n = 5/group. (C) Quantitative analysis of NE and MPO positive cells in different groups. n = 5/group. (D) Quantitative analysis of CD16/32 positive microglia in different groups. n = 5/group. (E) Quantitative analysis of IL1β positive microglia in different groups. n = 5/group. (F) Representative western blotting images of TNF-α expression in cortex in different groups. (G) Quantitative analysis of TNF-α expression in cortex in different groups. n = 6/group. (H) Representative western blotting images of IL1β expression in cortex in different groups. (I) Quantitative analysis of IL1β expression in cortex in different groups. n = 6/group. Scale bar = 50 μm. *P < 0.05 versus Sham, #P < 0.05 versus SAH + vehicle group.