Research Paper Volume 13, Issue 11 pp 14892—14909

Inhibition of CDK9 attenuates atherosclerosis by inhibiting inflammation and phenotypic switching of vascular smooth muscle cells

CDK9 inhibitor reduces HFD-induced inflammation phenotype switch of VSMCs in vivo. (A) TNF-α and IL-6 protein levels in the serum were detected using ELISA assay (n = 8; ###p *p **p B) TNF-α and IL-6 mRNA levels in the aorta were detected using real-time qPCR assay (n = 8; ##p ###p *p **p C–D) Western blot assay showed the expressions of α-SMA, Vimentin and PCNA in the whole aorta from ApoE-/- fed a normal (STD) or HFD for 16 weeks. Densitometric quantification for blots was shown in panel D (n = 8; ##p *p **p E) Representative immunofluorescence staining images for Vimentin (green) in aortic roots. Tissues were counterstained with DAPI (blue) (scale bar = 500 μm). (F) Representative images of PCNA staining of aortic roots (scale bar = 500 μm; DAB chromogen staining (brown)).

Figure 3. CDK9 inhibitor reduces HFD-induced inflammation phenotype switch of VSMCs in vivo. (A) TNF-α and IL-6 protein levels in the serum were detected using ELISA assay (n = 8; ###p < 0.001 compared to STD; *p < 0.05, **p < 0.01 compared to HFD). (B) TNF-α and IL-6 mRNA levels in the aorta were detected using real-time qPCR assay (n = 8; ##p < 0.01, ###p < 0.001 compared to STD; *p < 0.05, **p < 0.01 compared to HFD). (CD) Western blot assay showed the expressions of α-SMA, Vimentin and PCNA in the whole aorta from ApoE-/- fed a normal (STD) or HFD for 16 weeks. Densitometric quantification for blots was shown in panel D (n = 8; ##p < 0.01 compared to STD; *p < 0.05, **p < 0.01 compared to HFD). (E) Representative immunofluorescence staining images for Vimentin (green) in aortic roots. Tissues were counterstained with DAPI (blue) (scale bar = 500 μm). (F) Representative images of PCNA staining of aortic roots (scale bar = 500 μm; DAB chromogen staining (brown)).