Figure 2. C/EBPα transactivates NEAT1 through the -1453 and -54 sites in the NEAT1 promoter. (A) Schema of the NEAT1 promoter region shows the different mutation/truncation constructs used in this study. □ represents the wild-type C/EBP binding site and ⊠ represents the mutated site. (B) The wild-type (wt) or double mutated (co-mut) promoter construct (125 ng) was co-transfected into 293T cells along with the C/EBPα expression construct (500 ng). (C) Different mutation/truncation luciferase promoter plasmids were co-transfected with 500ng of the pcDNA3.1 (empty) or pcDNA3.1-C/EBPα (C/EBPα) vector into 293T cells. The data represent the mean ± S.E.M from three replicates. * indicates p<0.05.