Figure 5. Double knockdown of C/EBPα and C/EBPβ reduces ATRA-induced upregulation of C/EBPε and markedly impairs NEAT1 upregulation and NB4 cell differentiation. (A) C/EBPβ knockdown (kd-C/EBPβ) or control (NC) NB4 cells were transfected with C/EBPα siRNA (kd-C/EBPα) or negative control siRNA (NC). The protein levels of C/EBPα, C/EBPβ, C/EBPε, and GAPDH were determined in NB4 cells before and after ATRA treatment (1 μM for 24 h). (B) Expression of NEAT1 and NEAT1_2 isoform in C/EBPα and C/EBPβ double-silenced (double-KD) NB4 cells was analyzed after ATRA treatment for 24 h. (C) Flow cytometric analysis of CD11b, CD18, and CD11c expression in NB4 cells with or without C/EBPα and C/EBPβ double knockdown (double-KD) following ATRA treatment for 24h. The data represent the mean ± S.E.M. from three replicates. * indicates p<0.05.