Research Paper Volume 13, Issue 9 pp 13225—13238

TRIM14 regulates melanoma malignancy via PTEN/PI3K/AKT and STAT3 pathways

TRIM14 regulated melanoma tumorigenesis in vivo. A375 melanoma cells were transfected with si-TRIM14 or Oe-TRIM14 plasmid with or without pretreatment of LY2940/SH-4-54. After that, the above cells were injected into nude mice. The tumor was peeled off and weighed on day 20 to weigh and take pictures (A–B). The tumor growth curve for the animal experiment of each group was recorded and calculated (C). The tumor tissues were collected and applied to measure the protein expression of PCNA, Ki67, vimentin, and N-cadherin using western blot (D–E). *, ***p ###p

Figure 11. TRIM14 regulated melanoma tumorigenesis in vivo. A375 melanoma cells were transfected with si-TRIM14 or Oe-TRIM14 plasmid with or without pretreatment of LY2940/SH-4-54. After that, the above cells were injected into nude mice. The tumor was peeled off and weighed on day 20 to weigh and take pictures (AB). The tumor growth curve for the animal experiment of each group was recorded and calculated (C). The tumor tissues were collected and applied to measure the protein expression of PCNA, Ki67, vimentin, and N-cadherin using western blot (DE). *, ***p < 0.05, 0.001 vs control; ###p < 0.001 vs Oe-TRIM14.