Research Paper Volume 13, Issue 9 pp 13225—13238

TRIM14 regulates melanoma malignancy via PTEN/PI3K/AKT and STAT3 pathways

TRIM14 knockdown suppressed melanoma cell migration and invasion. After transfection in both WM451 and A375 cells, wound-healing assay and Transwell assay were applied to determine cell migration and invasion abilities, respectively (A–C). The protein expression of MMP2 and MMP9 in different groups of WM451 and A375 cells were measured using western blot (D–E). Magnification ×100. ***p

Figure 3. TRIM14 knockdown suppressed melanoma cell migration and invasion. After transfection in both WM451 and A375 cells, wound-healing assay and Transwell assay were applied to determine cell migration and invasion abilities, respectively (AC). The protein expression of MMP2 and MMP9 in different groups of WM451 and A375 cells were measured using western blot (DE). Magnification ×100. ***p < 0.001 vs siRNA-NC.