Figure 1. NEAT1 knockdown increases the inhibitory effect of anlotinib on A549 and NCI-H1975 cell viability. A549 and NCI-H1975 cells were transfected with siRNA-NC and NEAT1 siRNA 1, siRNA 2, or siRNA 3 for 24 h. NEAT1 levels were measured by RT-qPCR. (A) NEAT 1 levels in A549 cells and (B) NCI-H1975 cells. Cells were transfected with NEAT1 siRNA 3 (0, 5, 10, 20, 40 nM) for 24 h. (C) CCK-8 assays evaluated cell viability. (D–E) A549 and NCI-H1975 cells were treated with anlotinib or the combination of anlotinib and NEAT1 siRNA 3 for 24 h. (F) NCI-H1975 cells were treated with anlotinib (0, 10, 30, 40 μM) for 24 h and the level of NEAT1 was detected with RT-qPCR. **P < 0.01 compared with the control group.