Research Paper Volume 13, Issue 10 pp 13941—13953

NEAT 1 knockdown enhances the sensitivity of human non-small-cell lung cancer cells to anlotinib

NEAT1 knockdown increases the anti-invasion and anti-migration effect of anlotinib on A549 and NCI-H1975 cells. Cells were treated with anlotinib (10 μM), NEAT1 siRNA 3 (10 nM), or the combination treatment for 24 h. (A–B) Cell invasion assays were conducted on A549 and NCI-H1975 cells. Migrated cells were quantified. (C–D) Wound healing assays were performed in A549 and NCI-H1975 cells, and the wound healing rate was quantified. *P **P #P ##P

Figure 3. NEAT1 knockdown increases the anti-invasion and anti-migration effect of anlotinib on A549 and NCI-H1975 cells. Cells were treated with anlotinib (10 μM), NEAT1 siRNA 3 (10 nM), or the combination treatment for 24 h. (AB) Cell invasion assays were conducted on A549 and NCI-H1975 cells. Migrated cells were quantified. (CD) Wound healing assays were performed in A549 and NCI-H1975 cells, and the wound healing rate was quantified. *P < 0.05, **P < 0.01 compared with the control group. #P < 0.05, ##P < 0.01, compared with the anlotinib group.