Research Paper Volume 13, Issue 11 pp 14924—14939

Nucleolar small molecule RNA SNORA75 promotes endometrial receptivity by regulating the function of miR-146a-3p and ZNF23

SNORA75 promotes endometrial receptivity. (A) The effect of SNORA75 overexpression on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative PCR. (B) Western blotting was used to detect the effect of SNORA75 overexpression on the expression of receptivity-related factors in EEC cells. (C) Real-time quantitative fluorescence PCR was used to detect the effect of SNORA75 knockdown on the expression of receptivity-related factors in EEC cells. (D) Western blotting was used to detect the effect of SNORA75 knockdown on the expression of receptivity-related factors in EEC cells. (E) The effect of SNORA75 knockdown on LIF expression in EEC cell culture medium was detected by ELISA. (F) ELISA was used to detect the effect of SNORA75 overexpression on LIF expression in the culture medium of EEC cells. (G) Effect of SNORA75 silencing on the trophoblast adhesion rate. (H) Effect of SNORA75 overexpression on the trophoblast adhesion rate. (I) Effect of SNORA75 on embryo implantation. "##" indicates that, compared with the pLKO.1-vector group, P **" indicates that, compared with the pLVX-vector group, P

Figure 3. SNORA75 promotes endometrial receptivity. (A) The effect of SNORA75 overexpression on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative PCR. (B) Western blotting was used to detect the effect of SNORA75 overexpression on the expression of receptivity-related factors in EEC cells. (C) Real-time quantitative fluorescence PCR was used to detect the effect of SNORA75 knockdown on the expression of receptivity-related factors in EEC cells. (D) Western blotting was used to detect the effect of SNORA75 knockdown on the expression of receptivity-related factors in EEC cells. (E) The effect of SNORA75 knockdown on LIF expression in EEC cell culture medium was detected by ELISA. (F) ELISA was used to detect the effect of SNORA75 overexpression on LIF expression in the culture medium of EEC cells. (G) Effect of SNORA75 silencing on the trophoblast adhesion rate. (H) Effect of SNORA75 overexpression on the trophoblast adhesion rate. (I) Effect of SNORA75 on embryo implantation. "##" indicates that, compared with the pLKO.1-vector group, P < 0.01. "**" indicates that, compared with the pLVX-vector group, P < 0.01.