Figure 1. (A) Schematic representation of the compound testing for sirtuin activity evaluation in HDF. Cells were treated with or without the compounds for 24h and harvested for analysis. (B) Sirtuin activity assay results including H4K16 and H3K9 acetylation for cells treated with each compound. The results are shown by arrows that indicate increase/decrease (↑, ↓) and hyphens that indicate no effect (-). (C) Western Blot analysis and (D) quantification of H4K16ac and H3K9ac levels in cells treated with Resveratrol or Shikimic acid at the indicated concentrations. H4K16 and H3K9 levels were normalized to H4 and H3 levels respectively. Student T-test, *p<0.05, **p<0.01, ***p<0.001.