Research Paper Volume 13, Issue 9 pp 13287—13299

Exosomal circRNA 0001445 promotes glioma progression through miRNA-127-5p/SNX5 pathway

Glioma-derived exosomes promote glioma cell proliferation and migration. (A) The morphology and size distribution of EVs isolated from the supernatant of the culture medium of A172 and U373 cells, as detected by transmission electron microscopy and nanoparticle tracking analysis. scale bar = 100 nm. (B) Protein expressions of exosomal markers CD9, CD54, Annexin, and Calnexin in EVs and glioma cells. (C) Cell proliferation of glioma cells treated with exosomes isolated from A172 or U373 cells, as detected by CCK8 assay. (D) Wound healing assays in glioma cells treated with exosomes isolated from A172 or U373 cells. Scale bar = 200 μm. (E) Cell migration of glioma cells treated with exosomes isolated from A172 or U373 cells, as detected by Transwell invasion assay. Scale bar = 50 μm. Data were represented as mean ± SD. ** p

Figure 1. Glioma-derived exosomes promote glioma cell proliferation and migration. (A) The morphology and size distribution of EVs isolated from the supernatant of the culture medium of A172 and U373 cells, as detected by transmission electron microscopy and nanoparticle tracking analysis. scale bar = 100 nm. (B) Protein expressions of exosomal markers CD9, CD54, Annexin, and Calnexin in EVs and glioma cells. (C) Cell proliferation of glioma cells treated with exosomes isolated from A172 or U373 cells, as detected by CCK8 assay. (D) Wound healing assays in glioma cells treated with exosomes isolated from A172 or U373 cells. Scale bar = 200 μm. (E) Cell migration of glioma cells treated with exosomes isolated from A172 or U373 cells, as detected by Transwell invasion assay. Scale bar = 50 μm. Data were represented as mean ± SD. ** p < 0.01, *** p < 0.001.