Research Paper Volume 13, Issue 9 pp 13287—13299

Exosomal circRNA 0001445 promotes glioma progression through miRNA-127-5p/SNX5 pathway

miRNA-127-5p directly targets SNX5. (A) The putative binding sequence of miRNA-127-5p of 3′-UTR of SNX5, as predicted by online bioinformatics database StarBase and TargetScan. (B) Luciferase reporter assay in HEK293T cells co-transfected with miRNA-127-5p mimics or mimics negative control and reporter constructs carrying wild-type or mutant miRNA-127-5p binding sequence of 3′-UTR of SNX5. (C) The protein expression of SNX5 in glioma cells treated with miRNA-127-5p mimics or mimics negative control. (D) The mRNA expression of SNX5 in glioma cells treated with miRNA-127-5p mimics or mimics negative control. (E) The protein expression of SNX5 in glioma cells treated with exosomes isolated from A172 or U373 cells, exosomes isolated from A172 or U373 cells plus miRNA-127-5p mimics or mimics negative control. Cells in the control group received no treatment. (F) The mRNA expression of SNX5 in glioma cells treated with exosomes isolated from A172 or U373 cells, exosomes isolated from A172 or U373 cells plus miRNA-127-5p mimics or mimics negative control. Cells in the control group received no treatment. Data were represented as mean ± SD. ** p

Figure 5. miRNA-127-5p directly targets SNX5. (A) The putative binding sequence of miRNA-127-5p of 3′-UTR of SNX5, as predicted by online bioinformatics database StarBase and TargetScan. (B) Luciferase reporter assay in HEK293T cells co-transfected with miRNA-127-5p mimics or mimics negative control and reporter constructs carrying wild-type or mutant miRNA-127-5p binding sequence of 3′-UTR of SNX5. (C) The protein expression of SNX5 in glioma cells treated with miRNA-127-5p mimics or mimics negative control. (D) The mRNA expression of SNX5 in glioma cells treated with miRNA-127-5p mimics or mimics negative control. (E) The protein expression of SNX5 in glioma cells treated with exosomes isolated from A172 or U373 cells, exosomes isolated from A172 or U373 cells plus miRNA-127-5p mimics or mimics negative control. Cells in the control group received no treatment. (F) The mRNA expression of SNX5 in glioma cells treated with exosomes isolated from A172 or U373 cells, exosomes isolated from A172 or U373 cells plus miRNA-127-5p mimics or mimics negative control. Cells in the control group received no treatment. Data were represented as mean ± SD. ** p < 0.01, *** p < 0.001.