Research Paper Volume 13, Issue 9 pp 13287—13299

Exosomal circRNA 0001445 promotes glioma progression through miRNA-127-5p/SNX5 pathway

CircRNA 0001445 promotes glioma proliferation, migration, and invasion through miRNA-127-5p/SNX5 signaling pathway. (A) Cell proliferation of A172 cells treated with 1) control (no treatment); 2) exosomes isolated from A172 cells (A172-exo); 3) control plus circRNA 0001445 siRNA (hsa

Figure 6. CircRNA 0001445 promotes glioma proliferation, migration, and invasion through miRNA-127-5p/SNX5 signaling pathway. (A) Cell proliferation of A172 cells treated with 1) control (no treatment); 2) exosomes isolated from A172 cells (A172-exo); 3) control plus circRNA 0001445 siRNA (hsa_circ_0001445-si); 4) A172-exo plus hsa_circ_0001445-si; 5) A172-exo and hsa_circ_0001445-si plus miRNA-127-5p inhibitor (miRNA-127-5p-in); and 6) A172-exo and hsa_circ_0001445-si plus pcDNA3.1-SNX5, as detected by CCK8 assay. (B) Apoptosis of A172 cells treated as indicated in (A), as detected by flow cytometry analysis. (C) Cell migration of A172 cells treated as indicated in (A), as detected by Transwell invasion assay. Scale bar = 50 μm. (D) Wound healing assays in A172 cells treated, as indicated in (A). Scale bar = 200 μm. Data were represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.