Research Paper Volume 13, Issue 9 pp 13300—13317

SnoRD126 promotes the proliferation of hepatocellular carcinoma cells through transcriptional regulation of FGFR2 activation in combination with hnRNPK

C' box and D box are crucial for snoRD126 to promote cell proliferation. (A) qRT-PCR assay for snoRD126 expression in Huh7 cells with snoRD126 WT or mutants-overexpressing. (B) CCK8 assay performed in Huh7 cells with snoRD126 WT or mutants-overexpression. (C, D) Representative colony formation images and quantification of Huh7 cells with snoRD126 WT or mutants-overexpression. (E, F) Representative EdU images and quantification of Huh7 cells with snoRD126 WT or mutants-overexpression. Scale bar, 400um. (G) qRT-PCR assay for hnRNPK expression in HepG2 cells and snoRD126-overexpressing Huh7 cells with hnRNPK siRNA treatment. (H) CCK8 assays performed in snoRD126- or vector-overexpressed Huh7 cells with hnRNPK siRNA treatment. (I) Representative colony formation images and quantification of snoRD126- or vector-overexpressed Huh7 cells with hnRNPK siRNA treatment. The data represent mean ± SD (n = 3). *P

Figure 3. C' box and D box are crucial for snoRD126 to promote cell proliferation. (A) qRT-PCR assay for snoRD126 expression in Huh7 cells with snoRD126 WT or mutants-overexpressing. (B) CCK8 assay performed in Huh7 cells with snoRD126 WT or mutants-overexpression. (C, D) Representative colony formation images and quantification of Huh7 cells with snoRD126 WT or mutants-overexpression. (E, F) Representative EdU images and quantification of Huh7 cells with snoRD126 WT or mutants-overexpression. Scale bar, 400um. (G) qRT-PCR assay for hnRNPK expression in HepG2 cells and snoRD126-overexpressing Huh7 cells with hnRNPK siRNA treatment. (H) CCK8 assays performed in snoRD126- or vector-overexpressed Huh7 cells with hnRNPK siRNA treatment. (I) Representative colony formation images and quantification of snoRD126- or vector-overexpressed Huh7 cells with hnRNPK siRNA treatment. The data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01. NS, not significant.