Figure 7. Delay of autophagy onset in eIF2α.S52A cells during the aging process. (A) WT and eIF2α.S52 values were normalized A cells expressing CFP-Atg8 were grown in YES medium and harvested at different time points. Cell extracts were analyzed by immunoblotting using antibodies against CFP and Cdc2 (loading control). (B) Quantitative analysis of CFP-Atg8 cleavage in panel (A). The intensity of CFP band was quantified and the values normalized using Cdc2 as loading control. (C, D) Similar experiments were performed with cells grown in minimal medium without nitrogen (EMM-N). Actin was used as loading control. Data information: (A–D) Shown results are representative of at least three independent experiments.