Research Paper Volume 13, Issue 10 pp 14065—14077
TCF-3-mediated transcription of lncRNA HNF1A-AS1 targeting oncostatin M expression inhibits epithelial-mesenchymal transition via TGFβ signaling in gastroenteropancreatic neuroendocrine neoplasms
Figure 3. Knockdown of HNF1A-AS1 promoted cell viability. (A) qRT-PCR was performed to detect the best efficiency of siRNA-HNF1A-AS1-1, siRNA-HNF1A-AS1-2 and siRNA-HNF1A-AS1-3. Among them, siRNA-HNF1A-AS1-3 showed the best efficiency in both two cell lines. (B–C) CCK8 revealed that knockdown of HNF1A-AS1 induced increase of cell viability in both two cell lines. (D) The colony formation ability of GEP-NENs cells transfected with si-HNF1A-AS1 or negative control (si-NC) was determined. *p < 0.05, **p < 0.01.