Research Paper Volume 13, Issue 10 pp 14078—14087

Circular RNA ABCB10 contributes to laryngeal squamous cell carcinoma (LSCC) progression by modulating the miR-588/CXCR4 axis

CircABCB10 is able to sponge miR-588 in LSCC cells. (A) The potential interaction between circABCB10 and miR-588 was identified by the bioinformatic analysis using ENCORI (<a href="http://starbase.sysu.edu.cn/index.php" target="

Figure 3. CircABCB10 is able to sponge miR-588 in LSCC cells. (A) The potential interaction between circABCB10 and miR-588 was identified by the bioinformatic analysis using ENCORI (http://starbase.sysu.edu.cn/index.php). (BD) The AMC-HN-8 and Hep-2 cells were treated with the miR-588 mimic or control mimic. (B) The expression levels of miR-588 were measured by qPCR in the cells. (C) The luciferase activities of wild type circABCB10 (WT) and circABCB10 with the miR-588-binding site mutant (MUT) were determined by luciferase reporter gene assays in the cells. (E and F) The AMC-HN-8 and Hep-2 cells were treated with control shRNA or circABCB10 shRNA. The expression of miR-588 was analyzed by qPCR in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: **P < 0.01.