Research Paper Volume 13, Issue 9 pp 12334—12358

The nuclear sirtuin SIRT6 protects the heart from developing aging-associated myocyte senescence and cardiac hypertrophy

Sirt6.KO mice display cardiac aging phenotype. (A) Quantification of NAD+ contents in the heart lysate of wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (B) Relative Mitochondrial citrate synthase activity in the heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (C) Relative mitochondrial DNA lesions in the heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (D) 8-Oxo-dG content in the DNA of the whole heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (E) Relative telomere length of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (F) Heart lysates of Wild type and Sirt6.KO mice were subjected to immunoblotting using indicated antibodies. Representative blots of three different mice in each group are shown, n = 6. (Quantification of blots is given in Supplementary Figure 4A–4E).

Figure 3. Sirt6.KO mice display cardiac aging phenotype. (A) Quantification of NAD+ contents in the heart lysate of wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (B) Relative Mitochondrial citrate synthase activity in the heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (C) Relative mitochondrial DNA lesions in the heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (D) 8-Oxo-dG content in the DNA of the whole heart of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (E) Relative telomere length of Wild type and Sirt6.KO mice. Values are mean ± SE, n = 5. (F) Heart lysates of Wild type and Sirt6.KO mice were subjected to immunoblotting using indicated antibodies. Representative blots of three different mice in each group are shown, n = 6. (Quantification of blots is given in Supplementary Figure 4A4E).