Research Paper Volume 13, Issue 9 pp 12359—12377

Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization

MiR-142-3p is overexpressed in a laser-induced CNV mouse model. (A) Cryosections of control mouse (untreated) and laser-induced mouse delineating the different regions of interest (ROI). These ROIs, namely control choroid (in blue), adjacent choroid (in green) and CNV lesion (in orange), were isolated by laser microdissection. Scale bar = 250 μm. (B) MicroRNA expression in CNV lesion and adjacent choroid compared to control choroid, 7 days after laser induction. Results of CNV lesion and adjacent choroid are expressed as fold change to control choroid. Results are presented as mean +- SEM. One-way ANOVA followed by multiple comparisons test (n = 5 per experimental group). (C, D) Overexpression of miR-142-3p (C), and uPA (D), a CNV-disease marker, at day 5 (D5) and day 7 (D7). MiR-142-3p and uPA are specifically overexpressed in CNV lesion 5 and 7 days post laser induction. qRT-PCR results are presented as mean +- SEM. One-way ANOVA + multiple comparisons test (n = 10 for control choroid, n = 5 for adjacent choroid and CNV lesion D5, n = 10 for adjacent choroid and CNV lesion D7) (* = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001).

Figure 1. MiR-142-3p is overexpressed in a laser-induced CNV mouse model. (A) Cryosections of control mouse (untreated) and laser-induced mouse delineating the different regions of interest (ROI). These ROIs, namely control choroid (in blue), adjacent choroid (in green) and CNV lesion (in orange), were isolated by laser microdissection. Scale bar = 250 μm. (B) MicroRNA expression in CNV lesion and adjacent choroid compared to control choroid, 7 days after laser induction. Results of CNV lesion and adjacent choroid are expressed as fold change to control choroid. Results are presented as mean +- SEM. One-way ANOVA followed by multiple comparisons test (n = 5 per experimental group). (C, D) Overexpression of miR-142-3p (C), and uPA (D), a CNV-disease marker, at day 5 (D5) and day 7 (D7). MiR-142-3p and uPA are specifically overexpressed in CNV lesion 5 and 7 days post laser induction. qRT-PCR results are presented as mean +- SEM. One-way ANOVA + multiple comparisons test (n = 10 for control choroid, n = 5 for adjacent choroid and CNV lesion D5, n = 10 for adjacent choroid and CNV lesion D7) (* = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001).