Research Paper Volume 13, Issue 10 pp 14185—14197

Circular RNA ITCH promotes extracellular matrix degradation via activating Wnt/β-catenin signaling in intervertebral disc degeneration

CircITCH inhibits the proliferation and induces apoptosis of NP cells. (A) The expression levels of circITCH were measured by qPCR in the NP tissues of IDD patients (n=90) and normal cases (n=90). (B–F) The NP cells were infected with lentiviral plasmids carrying circITCH shRNA or corresponding control shRNA or transfected with the pcDNA3.1 or the pcDNA3.1-circITCH overexpression vector. (B) The expression levels of circITCH were examined by qPCR in the cells. (C, D) The cell proliferation was analyzed by CCK-8 assays in the cells. (E) Cell apoptosis was tested by flow cytometry analysis in the cells. (F) The expression of Bax, caspase3, cleaved caspase3 (c-caspase3), caspase9, and cleaved caspase9 (c-caspase9) was measured by Western blot analysis. Data are presented as mean ± SD. Statistic significant differences were indicated: * P

Figure 1. CircITCH inhibits the proliferation and induces apoptosis of NP cells. (A) The expression levels of circITCH were measured by qPCR in the NP tissues of IDD patients (n=90) and normal cases (n=90). (BF) The NP cells were infected with lentiviral plasmids carrying circITCH shRNA or corresponding control shRNA or transfected with the pcDNA3.1 or the pcDNA3.1-circITCH overexpression vector. (B) The expression levels of circITCH were examined by qPCR in the cells. (C, D) The cell proliferation was analyzed by CCK-8 assays in the cells. (E) Cell apoptosis was tested by flow cytometry analysis in the cells. (F) The expression of Bax, caspase3, cleaved caspase3 (c-caspase3), caspase9, and cleaved caspase9 (c-caspase9) was measured by Western blot analysis. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05.