Figure 1. CircITCH inhibits the proliferation and induces apoptosis of NP cells. (A) The expression levels of circITCH were measured by qPCR in the NP tissues of IDD patients (n=90) and normal cases (n=90). (B–F) The NP cells were infected with lentiviral plasmids carrying circITCH shRNA or corresponding control shRNA or transfected with the pcDNA3.1 or the pcDNA3.1-circITCH overexpression vector. (B) The expression levels of circITCH were examined by qPCR in the cells. (C, D) The cell proliferation was analyzed by CCK-8 assays in the cells. (E) Cell apoptosis was tested by flow cytometry analysis in the cells. (F) The expression of Bax, caspase3, cleaved caspase3 (c-caspase3), caspase9, and cleaved caspase9 (c-caspase9) was measured by Western blot analysis. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05.