Research Paper Volume 13, Issue 10 pp 14258—14276

LncRNA LOC100129620 promotes osteosarcoma progression through regulating CDK6 expression, tumor angiogenesis, and macrophage polarization

LncRNA LOC100129620 overexpression promotes the proliferation and invasion of osteosarcoma cells. (A) The expression of LOC100129620 in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (B) CCK-8 assay to analyze the viability of U2OS cells transfected with pLVX-Vector or pLVX-LOC100129620. (C) CCK-8 assay to analyze the viability of MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (D) Colony formation assay to analyze the proliferation of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (E) Quantitative analysis of colony formation of U2OS and MG63 cells with transfected pLVX-Vector or pLVX-LOC100129620. (F) Flow cytometry analysis of the cell cycle in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (G) Flow cytometry analysis of apoptosis of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (H) Cell scratch assay to detect the migration ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (I) Transwell assay to detect the invasion ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (J) Quantitative analysis of the Transwell assay results of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. Statistical analysis was conducted using Student’s t-test. Values are means ± SD. *P P

Figure 2. LncRNA LOC100129620 overexpression promotes the proliferation and invasion of osteosarcoma cells. (A) The expression of LOC100129620 in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (B) CCK-8 assay to analyze the viability of U2OS cells transfected with pLVX-Vector or pLVX-LOC100129620. (C) CCK-8 assay to analyze the viability of MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (D) Colony formation assay to analyze the proliferation of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (E) Quantitative analysis of colony formation of U2OS and MG63 cells with transfected pLVX-Vector or pLVX-LOC100129620. (F) Flow cytometry analysis of the cell cycle in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (G) Flow cytometry analysis of apoptosis of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (H) Cell scratch assay to detect the migration ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (I) Transwell assay to detect the invasion ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (J) Quantitative analysis of the Transwell assay results of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. Statistical analysis was conducted using Student’s t-test. Values are means ± SD. *P < 0.05 and **P < 0.01.