Figure 4. MEG8 sponged miR-454-3p in 293T cells. (A) The predicted binding site between MEG8 and miR-454-3p. (B) The predicted miR-454-3p binding site (MEG8-wt) and its matched mutant site (MEG8-mut). (C) Luciferase activity detection. (D, E) RT-qPCR analyses measured relative MEG8, miR-454-3p and TNF-α expression levels. (F) Elisa analyses measured TNF-α expression levels. (G) RT-qPCR analyses measured TNF-α expression levels. (H) Scanning electron microscope results of bone slices (500x). (I) Volume of resorption/pit (x103 um3). *P < 0.05, **P < 0.01, ***P < 0.001.