Research Paper Volume 13, Issue 11 pp 14968—14988

Construction of a novel mRNA-miRNA-lncRNA network and identification of potential regulatory axis associated with prognosis in colorectal cancer liver metastases

Correlation analysis among lncRNAs, APOC1 and hsa-miR-335-5p. (A) Correlation analysis between AC108488.4 and APOC1 in TCGA project. (B) Correlation analysis between ZEB1-AS1 and APOC1 in TCGA project. (C) Correlation analysis between ZEB1-AS1 and hsa-miR-335 in TCGA project. (D) Predicted binding sites in ZEB1-AS1 and APOC1 for miR-335-5p. (E) Luciferase reporter assay in HCT116 cells co-transfected with wide type (WT) or mutated (Mut) APOC1 3'-UTR reporter vector and miR-335-5p mimics or NC mimics. (F) Luciferase reporter assay in HCT116 cells co-transfected with wide type (WT) or mutated (Mut) ZEB1-AS1 reporter vector and miR-335-5p mimics or NC mimics. *p

Figure 6. Correlation analysis among lncRNAs, APOC1 and hsa-miR-335-5p. (A) Correlation analysis between AC108488.4 and APOC1 in TCGA project. (B) Correlation analysis between ZEB1-AS1 and APOC1 in TCGA project. (C) Correlation analysis between ZEB1-AS1 and hsa-miR-335 in TCGA project. (D) Predicted binding sites in ZEB1-AS1 and APOC1 for miR-335-5p. (E) Luciferase reporter assay in HCT116 cells co-transfected with wide type (WT) or mutated (Mut) APOC1 3'-UTR reporter vector and miR-335-5p mimics or NC mimics. (F) Luciferase reporter assay in HCT116 cells co-transfected with wide type (WT) or mutated (Mut) ZEB1-AS1 reporter vector and miR-335-5p mimics or NC mimics. *p < 0.05, **p < 0.01, *** p < 0.001.