Research Paper Volume 13, Issue 10 pp 14416—14432

miR-485 inhibits histone deacetylase HDAC5, HIF1α and PFKFB3 expression to alleviate epilepsy in cellular and rodent models

miR-485 repressed apoptosis, oxidative stress and inflammation by targeting HDAC5 in epilepsy model hippocampal neurons. (A) miR-485 and HDAC5 mRNA expression. (B) Binding relationship between miR-485 and HDAC5 determined by dual luciferase reporter gene assay. (C) Efficiency of HDAC5 knockdown. (D) HDAC5 protein expressions determined by western blotting. (E) Cell apoptosis determined by flow cytometry. (F) SOD levels. (G) GSH-Px levels. (H) MDA levels. (I) DNA 8-OHdG levels. (J) IL-1β, TNF-α, IL-6 levels. Data are expressed as mean ± standard deviation. * p p &p

Figure 2. miR-485 repressed apoptosis, oxidative stress and inflammation by targeting HDAC5 in epilepsy model hippocampal neurons. (A) miR-485 and HDAC5 mRNA expression. (B) Binding relationship between miR-485 and HDAC5 determined by dual luciferase reporter gene assay. (C) Efficiency of HDAC5 knockdown. (D) HDAC5 protein expressions determined by western blotting. (E) Cell apoptosis determined by flow cytometry. (F) SOD levels. (G) GSH-Px levels. (H) MDA levels. (I) DNA 8-OHdG levels. (J) IL-1β, TNF-α, IL-6 levels. Data are expressed as mean ± standard deviation. * p < 0.05 vs. blank cells, cells treated with mimic-NC, or epilepsy cells treated with sh-NC; # p < 0.05 vs. epilepsy cells treated with mimic-NC + oe-NC; &p < 0.05 vs. epilepsy cells treated with miR-485 mimic + oe-NC. Data between two groups were compared independent sample t-test. Data among multiple groups were compared by one-way analysis of variance and post-hoc Tukey’s test. Experiments were repeated three times.