Figure 2. Expression of miR-22-3p in HCC tissues and cell lines with or without alcohol exposure. (A) ISH detection of miR-22-3p in adjacent and cancerous tissues of HCC patients with or without alcohol consumption history. Original magnification, ×400. (B) The miR-22-3p expression level in (A) is quantified and shown in column graph. n= 25. (C) Relative miR-22-3p level in HCC cells and normal liver cells. **P< 0.01, n= 3. (D) Relative expression level of miR-22-3p in HepG2 cells treated with alcohol for 0, 14 or 21 days. (E) Relative expression level of miR-22-3p in SMMC-7721 cells treated with alcohol for 0, 14 or 21 days.