Figure 5. Effect of USP8 inhibition on receptor tyrosine kinase signaling in HCC cells. Representative image (A) and quantification (B, C) of western blots analysis of p-c-Met(Y1234/1235), c-Met, p-EGFR(Y1086), EGFR, p-Akt (S437), Akt, p-Stat3(T705), Stat3, p-Raf (S338/T341) and Raf in HuH6 cells after USP8 inhibition. si-Ctrl and control value were set as 1. Western blot was analysed after 24-hour drug treatment in HuH6 cells or at 72-hour post-transfection in siRNA transfected cells. *p<0.01, compared to si-Ctrl or control.