Research Paper Volume 13, Issue 11 pp 14999—15012

Inhibiting USP8 overcomes hepatocellular carcinoma resistance via suppressing receptor tyrosine kinases

Effect of USP8 inhibition on receptor tyrosine kinase signaling in HCC cells. Representative image (A) and quantification (B, C) of western blots analysis of p-c-Met(Y1234/1235), c-Met, p-EGFR(Y1086), EGFR, p-Akt (S437), Akt, p-Stat3(T705), Stat3, p-Raf (S338/T341) and Raf in HuH6 cells after USP8 inhibition. si-Ctrl and control value were set as 1. Western blot was analysed after 24-hour drug treatment in HuH6 cells or at 72-hour post-transfection in siRNA transfected cells. *p

Figure 5. Effect of USP8 inhibition on receptor tyrosine kinase signaling in HCC cells. Representative image (A) and quantification (B, C) of western blots analysis of p-c-Met(Y1234/1235), c-Met, p-EGFR(Y1086), EGFR, p-Akt (S437), Akt, p-Stat3(T705), Stat3, p-Raf (S338/T341) and Raf in HuH6 cells after USP8 inhibition. si-Ctrl and control value were set as 1. Western blot was analysed after 24-hour drug treatment in HuH6 cells or at 72-hour post-transfection in siRNA transfected cells. *p<0.01, compared to si-Ctrl or control.